E-MTAB-6265 - Transcription profiling by RNA-seq of murine colon tumours after in situ depletion of Nuak1

Port, J., Hedley, A. and Murphy, D. (2018) E-MTAB-6265 - Transcription profiling by RNA-seq of murine colon tumours after in situ depletion of Nuak1. [Data Collection]

Collection description

The aim of the project is to determine the impact of NUAK1 depletion on the transcriptome of in situ colon tumours. Tumours were initiated in Villin-CRE-ER;APC-floxed;DI-shNUAK1 mice, followed by treatment for 1 week with dextran sodium sulphate. 68 days after tumour initiation, NUAK1 shRNAs were induced by doxycycline administration (via gavage), or left un-induced. 6 tumours were harvested from NUAK1 shRNA expressing mice and 6 from controls. RNA was isolated using Qiagen RNEasy and analyzed by Illumina paired-end RNA-SEQ

Funding:
College / School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Date Deposited: 28 Mar 2019 11:55
URI: https://researchdata.gla.ac.uk/id/eprint/763

Available Files

There are no files for this dataset available to download.

Repository Staff Only: Update this record

Port, J., Hedley, A. and Murphy, D. (2018); E-MTAB-6265 - Transcription profiling by RNA-seq of murine colon tumours after in situ depletion of Nuak1

ArrayExpress

https://researchdata.gla.ac.uk/763

Retrieved: 2024-12-12