Port, J., Hedley, A. and Murphy, D. (2018) E-MTAB-6265 - Transcription profiling by RNA-seq of murine colon tumours after in situ depletion of Nuak1. [Data Collection]

Collection description

The aim of the project is to determine the impact of NUAK1 depletion on the transcriptome of in situ colon tumours. Tumours were initiated in Villin-CRE-ER;APC-floxed;DI-shNUAK1 mice, followed by treatment for 1 week with dextran sodium sulphate. 68 days after tumour initiation, NUAK1 shRNAs were induced by doxycycline administration (via gavage), or left un-induced. 6 tumours were harvested from NUAK1 shRNA expressing mice and 6 from controls. RNA was isolated using Qiagen RNEasy and analyzed by Illumina paired-end RNA-SEQ

Funding:	European Commission (EC) [618448] European Commission (EC) [705190] British Lung Foundation (BLF) [APHD13-5]
College / School:	College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Date Deposited:	28 Mar 2019 11:55
URI:	https://researchdata.gla.ac.uk/id/eprint/763
Additional details Additional details Dataset URL: https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-6265/ Type of data: Other Date: 19 February 2018 Date type: Publication Additional information: This is a metadata-only record. To access the data associated with this record, please follow the link provided to ArrayExpress, record accession E-MTAB-6265 Resource language: English Status: Published Data Publisher: ArrayExpress Last Modified: 28 Mar 2019 11:55

Repository Staff Only: Update this record