Qi, Y., Montague, P., Loney, C., Campbell, C., Shafie, I., Anderson, T. and McLaughlin, M. (2019) In vitro evidence consistent with an interaction between wild type and a mutant SOD1 protein associated with canine degenerative myelopathy. [Data Collection]
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Collection description
The data sets are associated with four experimental approaches to investigate a mutant gene that had been cloned into a vector plasmid to generate fluorescent tagged (Cherry or GFP wildtype or mutant SOD1 proteins:
a. a collection of fluorescent images of cells to count aggregate positive cells (WT and Mutant (DM) with GFP or Cherry) (JPEGS format)
b. A collection of western blots that confirm the expression of the SOD1 fusion protein, the fluorescent protein only and loading control with b-actin. (Tiff format)
c. A native gel treated with NBT to display SOD1 activity. Corresponding western blots shown to confirm SOD1 fusion protein expression. (Tiff format)
d. Confocal images showing the co-expression of wild type and mutant fusion proteins within cellular aggregates. (Tiff format)
e. Western blot of immunoprecipitation (pull down) products supporting the co-precipitation of wildtype and mutant SOD1. (Tiff format)
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