Garber, A. , Hastie, P. , Farci, V., Mcguinness, D. , Bulmer, L., Alzahal, O. and Murray, J. (2019) Yeast effect on faecal microbiome in ponies. [Data Collection]
Related Enlighten Publications
Collection description
The aim of the study was to determine the effect of a VistaEQ product containing 4% live yeast Saccharomyces cerevisiae strain Y1242 on faecal microbial populations when supplemented to high-starch and high-fibre diets using Illumina next generation sequencing of the V3-V4 region of the 16S rRNA gene. Seven mature Welsh ponies were used in a complete randomised 4 x 4 Latin square design consisting of 4 x 19-day periods. After DNA extractions from faeces and library preparation, alfa-diversity and Least disciminate analysis effect size (LEfSe) analysis were performed using 16S metagenomics pipeline in QIIME v1.9.0. Differences between the groups were considered significant when linear discriminant analysis (LDA) score >2. The study results showed that the Saccharomyces cerevisiae was able to induce positive changes in the equine microbiota when supplemented to a high-fibre diet: it increased relative abundance (RA) of members of Lachnospiraceae and Dehalobacteriaceae families, associated with a healthy core microbiome. Moreover, yeast supplementation increased the counts of fibrolytic bacteria (Ruminococcus) when fed with a high-fibre diet and reduced counts of lactate producing bacteria (Streptococcus) when a high-starch diet was fed. In addition, yeast increased RA of acetic, succinic acid producing bacterial family (Succinivibrionaceae) and butyrate producing bacterial genus (Roseburia) when fed with high-starch and high-fibre diets, respectively.
| College / School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health and Veterinary Medicine College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry and Nursing |
|---|---|
| Date Deposited: | 26 Aug 2019 15:08 |
| Related resources: | |
| URI: | https://researchdata.gla.ac.uk/id/eprint/873 |
Available Files
Data
Documentation
Read me
Repository Staff Only: Update this record