Fibroblast growth factor signaling in multiple sclerosis: inhibition of myelination and induction of pro-inflammatory signalling environment by Fibroblast Growth Factor 9.

Linington, C. , Lindner, M. and Thuemmler, K. (2015) Fibroblast growth factor signaling in multiple sclerosis: inhibition of myelination and induction of pro-inflammatory signalling environment by Fibroblast Growth Factor 9. [Data Collection]

Original publication URL: http://dx.doi.org/10.1093/brain/awv102

Collection description

Analysis of the effects of three members of the FGF family (FGF1, FGF2 and FGF9) and bone morphogenic protein 4 (BMP4) on myelinating cultures generated from dissociated embryonic spinal cord. The results of both immediate (24 hours, T1 (24 hrs)) and long term treatments (10days, T2) give insights into the cumulative effects of sustained FGF and BMP mediated signal transduction in the pathogenesis of demyelinating diseases.

Dissociated myelinating cultures were generated from neurosphere derived astrocytes (generated from striata of P1 Sprague-Dawley rats) and spinal cord cells from E15.5 Sprague-Dawley(SD) embryos. The effect of recombinant human FGF9 and other soluble factors on these in vitro myelinating cultures was investigated by adding them to the culture media after 18 days in vitro (DIV). The effects of each factor were analysed using three independent cultures at two time points (19DIV and 28 DIV).

Funding:
Keywords: Expression profiling, Rattus norvegicus
College / School: College of Medical Veterinary and Life Sciences > School of Infection and Immunity > Immunology
Date Deposited: 21 Apr 2015 09:45
Enlighten Publications URL: http://eprints.gla.ac.uk/105402/
URI: http://researchdata.gla.ac.uk/id/eprint/160

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Linington, C. , Lindner, M. and Thuemmler, K. (2015); Fibroblast growth factor signaling in multiple sclerosis: inhibition of myelination and induction of pro-inflammatory signalling environment by Fibroblast Growth Factor 9.

University of Glasgow

DOI: 10.5525/gla.researchdata.160

Retrieved: 2022-08-09