Yeast effect on faecal microbiome in ponies

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http://eprints.org/relation/isVersionOf https://researchdata.gla.ac.uk/id/document/3019 http://eprints.org/relation/isVolatileVersionOf https://researchdata.gla.ac.uk/id/document/3019 http://eprints.org/relation/issmallThumbnailVersionOf https://researchdata.gla.ac.uk/id/document/3019 archive 5 disk0/00/00/08/73 2019-08-26 15:08:14 2022-11-11 09:53:03 2019-08-26 15:08:14 data_collection show Garber Anna 32885 0000-0001-8762-7030 Hastie Peter 10279 0000-0001-7845-0114 Farci Viola 45355 NO-ORCID Mcguinness David 34150 0000-0003-2947-2047 Bulmer Louise 41896 Alzahal O. Murray Juliette 34858 glaresearchdata:2019-08-26-873 Yeast effect on faecal microbiome in ponies pub 20000000 20300000 20200000 Cites funding from AB Vista, grant ref: 172757-01 The aim of the study was to determine the effect of a VistaEQ product containing 4% live yeast Saccharomyces cerevisiae strain Y1242 on faecal microbial populations when supplemented to high-starch and high-fibre diets using Illumina next generation sequencing of the V3-V4 region of the 16S rRNA gene. Seven mature Welsh ponies were used in a complete randomised 4 x 4 Latin square design consisting of 4 x 19-day periods. After DNA extractions from faeces and library preparation, alfa-diversity and Least disciminate analysis effect size (LEfSe) analysis were performed using 16S metagenomics pipeline in QIIME v1.9.0. Differences between the groups were considered significant when linear discriminant analysis (LDA) score >2. The study results showed that the Saccharomyces cerevisiae was able to induce positive changes in the equine microbiota when supplemented to a high-fibre diet: it increased relative abundance (RA) of members of Lachnospiraceae and Dehalobacteriaceae families, associated with a healthy core microbiome. Moreover, yeast supplementation increased the counts of fibrolytic bacteria (Ruminococcus) when fed with a high-fibre diet and reduced counts of lactate producing bacteria (Streptococcus) when a high-starch diet was fed. In addition, yeast increased RA of acetic, succinic acid producing bacterial family (Succinivibrionaceae) and butyrate producing bacterial genus (Roseburia) when fed with high-starch and high-fibre diets, respectively. 2019-08-26 published University of Glasgow 10.5525/gla.researchdata.873 Spreadsheet Text TRUE EN 2016-10-10 2017-01-15 https://theses.gla.ac.uk/83258/ thesis 2029-08-26 R TRUE 05a/14 mvls FALSE The effect of supplementing pony diets with yeast on 2. The faecal microbiome. http://eprints.gla.ac.uk/id/eprint/220200