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ReadMe.txt

readme 884 3 5165 document 884 Dataset.zip application/x-zip 4b4f4764f7ed31d7b1bd7d92db2f91b2 MD5 408969811 2016-08-01 09:56:56 https://researchdata.gla.ac.uk/332/2/Dataset.zip 332 2 2 application/x-zip Spreadsheet en public cc_by_sa_4

Dataset.zip

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http://eprints.org/relation/isVersionOf https://researchdata.gla.ac.uk/id/document/883 http://eprints.org/relation/isVolatileVersionOf https://researchdata.gla.ac.uk/id/document/883 http://eprints.org/relation/isIndexCodesVersionOf https://researchdata.gla.ac.uk/id/document/883 archive 10322 disk0/00/00/03/32 2016-07-12 14:34:38 2025-05-22 10:27:38 2016-08-01 10:15:18 data_collection show Ngandu Mpoyi Elie 41486 Cantini Marco 30437 0000-0003-0326-1508 Reynolds Paul 15349 0000-0001-7614-0008 Gadegaard Nikolaj 10390 0000-0002-3396-846X Dalby Matthew 9268 0000-0002-0528-3359 Salmerón-Sánchez Manuel 30067 glaresearchdata:2016-07-08-332 pub 25400000 30303000 Surface nanotopography is widely employed to control cell behavior and in particular controlled disorder has been shown to be important in cell differentiation/maturation. However, extracellular matrix proteins, such as fibronectin (FN), initially adsorbed on a biomaterial surface are known to mediate the interaction of synthetic materials with cells. In this work, we examine the effect of nanotopography on cell behavior through this adsorbed layer of adhesive proteins using a nanostructured polycarbonate surface comprising 150 nm-diameter pits originally defined using electron beam lithography. We address the effect of this nanopitted surface on FN adsorption and subsequently on cell morphology and behavior using C2C12 myoblasts. Wettability measurements and atomic force microscopy imaging showed that protein is adsorbed both within the interpits spaces and inside the nanopits. Cells responded to this coated nanotopography with the formation of fewer but larger focal adhesions and by mimicking the pit patterns within their cytoskeleton, nanoimprinting, ultimately achieving higher levels of myogenic differentiation compared to a flat control. Both focal adhesion assembly and nanoimprinting were found to be dependent on cell contractility and are adversely affected by the use of blebbistatin. Our results demonstrate the central role of the nanoscale protein interface in mediating cell-nanotopographical interactions and implicate this interface as helping control the mechanotransductive cascade. The dataset contains data files with original raw data and descriptions of how they were processed. The dataset has been created to help anyone interested in the work carried out in this paper to view and understand the data. 2016-07-08 published University of Glasgow 10.5525/gla.researchdata.332 Spreadsheet University of Glasgow 62690 1 HEALINSYNERGY - Material-driven fibronectin fibrillogenesis to engineer synergistic growth factor microenvironments Manuel Salmeron-Sanchez European Research Council (ERC) 306990 ENG - BIOMEDICAL ENGINEERING 65373 1 Synergistic microenvironments for non-union bone defects Matthew Dalby Medical Research Council (MRC) MR/L022710/1 RI MOLECULAR CELL & SYSTEMS BIOLOGY 47056 3 DTC in cell and proteomic technologies (continuation) Jonathan Cooper Engineering & Physical Sciences Research Council (EPSRC) EP/F500424/1 ENG - BIOMEDICAL ENGINEERING FALSE EN 2014-03 2014-04 http://dx.doi.org/10.1021/acsnano.6b01649 article 2026-07-08 R FALSE FALSE http://eprints.gla.ac.uk/id/eprint/120956