This data is from a study designed to investigate the role of AMPK in endothelium-dependent anticontractile effects of PVAT and which PVAT-derived factors could be involved. It contains data on vascular function using myography to measure contraction and relaxation of mouse aortic rings from wild-type (WT) and global AMPKα1 knockout (KO) aortic rings. It also has secretory function of aortic PVAT data from an immunoblotting array and ELISA, and gene expression data using qPCR. Human Umbilical Vein Endothelial Cells (HUVECs) and 3T3-L1 adipocytes how adding conditioned media (CM) derived from WT and KO PVAT affects eNOS expression, and nitric oxide (NO) release as well as AMPK-dependant pathways.