This dataset comprises of in-depth, semi-structured interviews, undertaken with employees at various levels, from five Scottish organisations. This includes four businesses and one social enterprise. The case studies were selected, due to their self-identification as being purpose informed organisations. The interviews were undertaken with participants employed in various roles and positions, ranging from warehouse workers to CEO’ s and business owners. The interviews were all undertaken online via the video application Microsoft Teams. The dataset informed the qualitative research project, ‘Purpose in Business: exploring the relationship between purpose and productivity’ which was funded by the ESRC and the Productivity Institute, with the support of Prosper.