Data was gathered during 2021 from 76 sheep farmers across Scotland, focusing on their use and understanding of the ectoparasiticide, moxidectin in their flock. In addition, it contains data from post-moxidectin treatment sheep faecal samples collected from sheep treated by the farmer on six separate farms across Scotland. From each farm two sets of samples were collected, and the data contains information on the faecal egg counts and species identified using PCR of the ITS2 region. The data may be viewed and re-used by others.