1. Paper device folding and component 1.1 component 1.2 paper folding steps 2. DNA recovery with real-time PCR 2.1 DNA extraction 2.2 serial dilution samples 2.3 standard curve 3. International stanard sample sensitivity test-normalized 4. Parasites sensitivity test 5. Clinical trail results 5.1 Raw pictures 5.2 Clinical trial results analysis 6. Samples treated with different methods 6.1 Raw picture 6.2 Data analysis