Three 40uM under-agarose experiments were preformed in parallel and, after ~11h were filmed for a short time at 10x in phase contrast. They were then each chopped into 1.1mm sections, starting at the well edge, and each section was placed in a methanol/ethanol solvent in order to allow remaining folate to dissolve. Each sample was kept rotating at 4"C for 1h 30min before being frozen at -80"C for storage and, when schedules permitted, taken to Mass Spec for analysis.