README – TFB PET DICOM Dataset

1. Dataset Overview
This dataset contains PET DICOM imaging studies acquired as part of the project “Multi-scale in vivo imaging of tumour evolution using a germline conditional triple-reporter mouse”. The scans include [18F]tetrafluoroborate (TFB) PET with MRI for anatomical co‑registration. Studies cover both liver and lung tumour models in conditional genetically engineered mouse strains harbouring the Rosa26LSL‑NRL triple-reporter allele.
All files are provided in anonymised DICOM format with acquisition dates and institutional metadata preserved.

2. Contents Summary
The DICOM server export includes two main categories of imaging sessions:
A. Liver PET studies
Typical study descriptions include:


“LIVER PD – TFB PET – GM – UBMX2.2f”
Date: 2022‑09‑19
Modality: TFB PET + Liver MRI
Operator: “Tom Bird – R09 – Liver MRI – SMB‑DMB”
Notes: Single‑mouse liver PET/MRI scan using the TFB reporter readout.


“LIVER LUNG TB_DYL–TFB PET–EB–AA‑UBMX4.1h–UAKA16.4b”
Date: 2024‑06‑13
Modality: Combined liver and lung PET/MRI session
Operator: “Tom Bird – R09 – Liver MRI – SMB‑DMB”
Multi‑bed acquisition allowing simultaneous capture of thoracic and abdominal structures.


B. Lung PET/MRI studies
Multiple lung imaging series are included, with typical naming patterns such as:

“LUNG_UNRLKP_CRE_TFB_PET_JO4_UAKA7.1c”
“LUNG_UNRLKP_TFBBED1_UAKA35.1d”
“LUNG_UNRLKP_TFB_PET_EB_UAKA47.3e–48.3f”

These series represent:

TFB PET‑MRI scans of NRL‑KP (Kras^G12D; Trp53^fl/fl; Rosa26LSL‑NRL) lung tumour models

Imaging dates spanning 2021–2024

All datasets correspond to individual animals imaged once per listed date.

3. Imaging Modalities and Protocols

Radiotracer: [18F]tetrafluoroborate (TFB), a PET tracer for mNIS reporter gene expression
Scanner: Mediso NanoScan PET/MR (1T)
PET acquisition: Static imaging, 70–90 minutes post‑injection
MRI: T1‑weighted anatomical sequence for co‑registration
Format: DICOM (PET and MRI datasets for each session)


4. Animal Models Represented
The imaging corresponds to mice bearing the following conditional alleles (various combinations across studies):

Rosa26LSL‑NRL (mNIS‑TagRFP‑Luc2 triple reporter)
KrasLSL‑G12D
Trp53fl/fl
Ctnnb1ex3
Rosa26LSL‑MYC

These models are used for:

Lung adenocarcinoma studies (NRL‑KP)
Hepatocellular carcinoma studies (NRL‑BM)
Ubiquitous Cre induction controls (NRL‑CV, NRL‑CE)


5. File Organisation
Each folder corresponds to one DICOM study and includes:

Full PET image set
DICOM header fields

Recommended usage: load in VivoQuant, Fiji, 3D‑Slicer, or any DICOM‑compatible viewer.

6. Ethical and License Notes

All imaging was performed under UK Home Office licences and institutional animal ethics approval.
The dataset is released under CC‑BY (unless Enlighten enforces a specific licence).


7. Citation
If reused, please cite the associated manuscript:
Dzien et al., “Multi-scale in vivo imaging of tumour evolution using a germline conditional triple‑reporter mouse”, Nature Biotechnology (2026).

8. Contact
For questions regarding dataset structure or additional metadata:
Prof. David Lewis
CRUK Scotland Institute
Email: david.lewis@glasgow.ac.uk