README: SLC5A5 / Luciferase RNAscope Block ID Dataset
1. Dataset Title
SLC5A5 and Luciferase RNAscope Block Identifiers 

This dataset accompanies the article:
“Multi‑scale in vivo imaging of tumour evolution using a germline conditional triple‑reporter mouse” (published in Nature Biotechnology).

2. Dataset Overview
This dataset contains the full table of RNAscope block identifiers used in the manuscript, including:

SLC5A5 (NIS) RNAscope imaging blocks
Luciferase RNAscope imaging blocks
Associated genotype information
Tissue type
Mouse IDs and colony codes
Vector type used (AAV‑CRE, AD5‑CRE, AAV‑EMPTY)
Whether blocks appear in paper figures, extended data, or supplementary analyses

The dataset serves as a lookup and metadata reference connecting each imaged tissue block to:

The biological model (mouse line, genotype)
The experimental manipulation (induction type)
The imaging readout (SLC5A5/NIS or luciferase expression)
The figure panels reported in the publication

This supports transparency, reproducibility, and data traceability across imaging analyses.

3. File List
SLC5A5_RNAscope_block_IDs.xlsx
Contains one worksheet:

Block IDs
Columns include:

Colony
Code
Mouse ID
Tissue
File number
Genotype (colony)
Genotype (NIS)
Induction
RNAscope probe used (NIS or Luciferase)
In paper analysis (figure reference)




4. Methods Summary
Mouse Lines & Genotypes
The dataset includes samples from:

UNRL‑BM (BM liver tumour/NIS‑reporter models)
UNRL‑KP (KP/Cre‑driven lung adenocarcinoma models)
NRL‑CE / NRL‑CV developmental reporter models

Genotypes listed in the file correspond to colony breeding records and reporter status.
Induction Methods

AAV‑CRE for hepatic or whole‑organ induction
AD5‑CRE for lung tumour initiation
AAV‑EMPTY controls
N/A where no viral induction was used (germline induction)

RNAscope Probes

SLC5A5 (NIS)
Luciferase

Tissues Included

Liver
Tumour (various)
Lung
Thyroid
Heart
Brain
Kidney
Pancreas
Spleen
Muscle
Others as listed in the file


5. Relation to the Published Article
Each block record includes a column indicating where it was used in the manuscript, e.g.:

Fig. 4f / 4g
Extended Data Fig. 5c
Extended Data Fig. 6b
Fig. 2f / 2g
Etc.

This allows users to trace specific tissue blocks back to the images shown in the paper.

6. Data Use & Licence
Unless specified otherwise by your deposit settings:

Licence: Creative Commons Attribution (CC‑BY)
The dataset may be reused, shared, and adapted with proper citation.

Recommended citation text can be generated if you provide the repository DOI.

7. Access Notes
All documentation and metadata included in this file contain no restricted information and are suitable for open sharing.
No personal data or regulated animal identifiers are included.

8. Contact
For questions regarding the dataset, imaging methods, or additional data availability, please contact:
David Lewis
Professor of Molecular Imaging
CRUK Scotland Institute, University of Glasgow
Email: davd.lewis@glasgow.ac.uk