The files in this folder are for the de-novo transcriptome in a paper published in Molecular Ecology (Strowbridge et al. 2026), which investigates how gene expression and m⁶A RNA methylation shape colour variation in the fire salamander, Salamandra salamandra bernardezi. Using long‑read PCR cDNA, the authors assembled a de novo transcriptome for use in colouration research within Salamandra.

The following provides a concise overview of each file, with a small description of what is contained in each file.

**rnabloom_transcriptome_kmer19.fasta**

Description: Fasta file of the de-novo transcriptome assembly, with transcript name, length, and coverage.

**rnabloom_transcriptome_kmer19.fasta.fai**

Description: Fasta index file, with length, offset, line bases and line width information.

**txname_all_annotations.csv**

Description: Annotation csv file with Transcript_ID, GENEID, Preferred_name, GOs, Description, KEGG_ko, KEGG_Pathway, KEGG_Module, KEGG_Reaction, KEGG_rclass, PFAMs.

**txname_all_annotations+blast.csv**

Description: Annotation csv file with Transcript_ID, GENEID, Preferred_name, GOs, Description, KEGG_ko, KEGG_Pathway, KEGG_Module, KEGG_Reaction, KEGG_rclass, PFAMs. Added blast annotated transcripts with Transcript_ID and Preferred_name.