1. Dataset Title

Sporosarcina pasteurii urease gene expression regulation mechanism overcomes urease inhibition by hydrocarbons

2. Authors / Creators
Name(s): Carla Comadran Casas
Affiliation(s): University of Glasgow
ORCID(s): https://orcid.org/0000-0003-0728-4395
Contact email: Carla.ComadranCasas@glasgow.ac.uk

3. Description

What the dataset contains: experimental data supporting article graphs and statistical analysis
Purpose of the data collection: study the molecular biology and biochemistry of S. pasteurii in hydrocarbon polluted environment to develop MICP applications in contaminated soils. 
Research context or project it supports: GALLANT Regenerating Vacant and Derelict Land team

4. Data Collection Details
Date(s) of data collection: 2025
Location: University of Glasgow
Methodology: laboratory experiments
Instruments / tools used: Flow cytometry (FCM), Spectrophotometry, quantitative Polymerase Chain Reaction (qPCR), pH, Fluorescence Excitation & Emission spectroscopy (FEEM)

5. File Overview

File Name	Description	Format
250904_NH4_SPHC2_R.xlsx	Ammonium (Spectrophotometry) and pH of liquid media	Excel
results_stats_NH4_tr.txt	results statistical analysis 250904_NH4_SPHC2_R.xlsx file	Text
COV14_SoilExtract_SPHC2_EEM.csv	FEEM analysis of HC1 media	CSV
SPHC2_ureC_DNA_RNA_metadata.xlsx	metadata of ureC gene abundance quantification by qPCR in DNA and RNA samples	Excel
SPHC2_ureC_RNA_cDNA_metadata.xlsx	metadata of ureC gene expression quantification by qPCR in cDNA samples	Excel
2025-09-019_162016_SPHC2_ureC_RNA_cDNA_Standard Curve Result_20250919_150816.csv	raw data qPCR analysis of ureC gene expression quantification by qPCR in cDNA samples	CSV
2025-09-02_162016_SPHC2_ureC_DNA_Standard Curve Result_20250903_100426.csv	raw data qPCR analysis of ureC gene abundance quantification by qPCR in DNA samples	CSV
2025-09-02_162016_SPHC2_ureC_RNA_Standard Curve Result_20250903_100438.csv	raw data qPCR analysis of ureC gene abundance quantification by qPCR in RNA samples	CSV
stats_qPCR.docx	results statistical analysis qPCR files listed above	Word
flow_ICC_R_170725.xlsx	results FCM intact cell count	Excel
flow_TCC_R_170725.xlsx	results FCM total cell count	Excel
Intactcountstats.txt	statistical analysis intact cell count FCM	text
Totalcountstats.txt	statistical analysis intact cell count FCM	text


6. Data Structure & Variables

Explain:

Variables:
Media: HC0, HC1, HC0.1, HC0.01 please refer to manuscript for detailed explanation 
Dil: dilution factor
Rep: biological replicate
NH4: ammonium
tr: reaction time (h)

In qPCR files:
Quantity (in qPCR files): raw analysis result 
initial vol sample (mL): volume of media in which S. pasteurii was grown
template: type of sample (i.e., DNA or RNA from extraction procedure)
vol extracted sample (uL): volume of sample obtained at the end of DNA/RNA extraction procedure

in FCM files:
cells (+/-/F): '+' means sample contained bacteria, '-' control samples (no bacteria), 'F' filtered samples (used for background checks)
BioRep: biological replicate
HC: media, where DI is deionised water (control)
time(h): reaction time
Dil: dilution factor of sample for FCM analysis.


Provide how users should cite the dataset:

Comadran-Casas, Carla (2026). Sporosarcina pasteurii urease gene expression regulation mechanism overcomes urease inhibition by hydrocarbons. University of Glasgow. DOI: http://dx.doi.org/10.5525/gla.researchdata.2150

12. Related Publications

https://doi.org/10.1016/j.jhazmat.2026.142148

13. Funding Information
Funder(s): Natural Environment Research Council as part of the Changing the Environment Programme 
Grant number(s):NE/W005042/1


14. Version Information
Version number: 1
Date released: 22/04/2026
Changes from previous versions:

15. Contact Information
Name: Carla Comadran Casas
Email: Carla.ComadranCasas@glasgow.ac.uk