README - Source Data for "SFTSV NSs protein is a tick antiviral RNAi response suppressor"
===========================================================================================

Dataset Contents
-----------------
This dataset contains the source data files for figures in the research article by Fares et al. (2025).

Files Included:
1. Fares_et_al_-_Underpinning_data.xlsx (1.2 MB)
   - Excel spreadsheet containing raw numerical data for all figures
   - 82 separate worksheets organised by figure panels

2. Fares_et_al_2025_-_Source_Data.docx (31 KB)
   - Microsoft Word document providing a comprehensive index
   - Contains a table mapping each worksheet tab to its corresponding figure panel
   - Includes descriptions and units of measurement for each dataset

Software Requirements
---------------------
To access the data files, you will need:

- Microsoft Excel (2010 or later) OR LibreOffice Calc OR any compatible spreadsheet application
  Required to open: Fares_et_al_-_Underpinning_data.xlsx

- Microsoft Word (2010 or later) OR LibreOffice Writer OR any compatible word processor
  Required to open: Fares_et_al_2025_-_Source_Data.docx

Data Organisation and File Structure
------------------------------------

Excel File Structure (Fares_et_al_-_Underpinning_data.xlsx):
The spreadsheet contains 82 worksheets organised using the following naming conventions:

1. MAIN FIGURES (46 worksheets):
   - Naming pattern: [Figure number][Panel letter]
   - Examples: 1A, 1B, 1C, ..., 7A, 7B
   - Covers data for Figures 1-7 in the main manuscript

2. SUPPLEMENTARY FIGURES (30 worksheets):
   - Naming pattern: S[Figure number][Panel letter]
   - Examples: S1A, S1B, S2A, ..., S12A-E
   - Covers data for Supplementary Figures S1-S12

3. EXPERIMENTAL REPLICATES (6 worksheets):
   - Naming pattern: S[Figure number]rep[Replicate number]
   - Examples: S8rep1, S8rep2, S8rep3, S9rep1, S9rep2, S9rep3
   - Contains individual replicate data for Supplementary Figures S8 and S9

Word Document Structure (Fares_et_al_2025_-_Source_Data.docx):
- Contains a single table with 86 rows (including header)
- Three columns: Tab | Figure | Description of data presented & Unit of measure
- Provides a complete cross-reference between worksheet tabs and figure panels
- Includes data descriptions and units of measurement for each dataset

Data Format and Content Notes
-----------------------------

General Data Format:
- Numerical data are presented as raw values (not formatted for display)
- Some worksheets contain multiple experimental conditions in separate columns
- Missing data points are represented as empty cells or "NaN" values
- Column headers may contain experimental condition labels (e.g., "WT MOI 0.1", "ΔNSs:eGFP MOI 1")

Common Abbreviations and Terminology:
- WT: Wild-type virus
- ΔNSs: NSs deletion mutant virus
- MOI: Multiplicity of infection
- FFU/ml: Focus-forming units per millilitre (virus titre measurement)
- eGFP: Enhanced green fluorescent protein
- S, M, L: Small, Medium, Large viral genome segments
- rep: Replicate experiment

Special Notes:
- Some worksheets may contain the notation "No associated raw data" for figure panels that are schematic or do not require numerical data
- Worksheet tabs with multi-panel designations (e.g., "S12A-E") contain data for multiple related panels
- Data for virus growth curves, protein expression levels, and molecular interactions are presented in various formats depending on the experimental approach
- Time-course data typically include time points in the first column with corresponding measurements in subsequent columns

Data Interpretation:
- Consult the Word document (Fares_et_al_2025_-_Source_Data.docx) for specific descriptions of what each dataset represents
- Units of measurement are provided in the Word document for each figure panel
- For questions about experimental methods or data interpretation, refer to the main manuscript and supplementary methods

Version Information:
- Files were last modified: 29 April 2026
- This README corresponds to the final published version of the research article

Contact Information:
For questions about this dataset, please contact the corresponding authors as listed in the published manuscript.