Title: Alignment-free construction of double emulsion droplet generation devices incorporating surface wettability transition control Authors: Yunus Aslan, Olivia McGleish, Julien Reboud, Jonathan M. Cooper This readme file aims to provide a guide to be used with the data associated with the aforementioned publication. Note: All files are provided as comma separated values (CSV) files in UTF-8 format and contain only single sheets. Any software can be used for replotting and analysis as appropriate. Any additional details pertinent to each file are detailed below. List of datasets: Fig 2(B) Fig 4(B) Fig 5(A) Fig 5(B) Fig 6 Fig S1 Fig S3 Fig S4 Dataset Details ‘Fig 2(B).csv’ contains the data associated with Fig. 2(B). Column 1 is the day, columns 2-16 are the measured contact angles for three measurements of each percentage w/w PDMS-Brij L4 mix: 0%, 0.25%, 0.75%, 1%, and 1.5%. The units of columns 2-16 is degrees. The mean and standard deviation for each percentage w/w PDMS-Brij L4 mix are provided in columns 18-27, also in degrees. Column naming conventions are the substrate, PDMS or PDMS/Brij L4 percentage, followed by the measurement number. Column 17 is intentionally left blank for readability. ‘Fig 4(B).csv’ contains the data associated with Fig. 4(B). Column 1 is the flow ratios (Qout/Qmid). Column 2 contains the droplet ID for the three repeated measurements. Columns 3-4 are the surface area (units squared metres) for the outer and inner droplets. The height of the droplets is given in column 5, also in squared metres. The volume calculations are contained in columns 6-9 for the outer and inner droplet, initially in metres cubed (columns 6 and 7) and then converted to nano Litres (columns 8 and 9). Finally, the mean and standard deviations of the droplet volumes are given in columns 12-15 with the Flow rates reprised in column 11 for ease of reading. Column 10 is intentionally left blank for readability. ‘Fig 5(A).csv’ contains the data associated with Fig. 5(A). Column 1 is the inner droplet diameter whereas column 2 contains the outer droplet diameter. Each column has units of micrometre. ‘Fig 5(B).csv’ contains the data associated with Fig. 5(B). Column 1 is the inner droplet diameter whereas column 2 contains the outer droplet diameter. Each column has units of micrometre. ‘Fig 6.csv’ contains the data associated with Fig. 6. Column 1 is the distance in micrometres for the line profile drawn across the individual droplets. Columns 2-6 contain the data for the fluorescence intensity profiles of five different liposomes shown in Fig. 6 with matching column labels, with arbitrary units. ‘Fig S1.csv’ contains the data associated with Fig. S1. Column 1 is the wavelength in nanometres. Columns 2-6 are the transmission percentages for PDMS, 0.25% w/w PDMS-Brij L4 mix, 0.75% w/w PDMS-Brij L4 mix, 1% w/w PDMS-Brij L4 mix, and 1.5% w/w PDMS-Brij L4 mix respectively. ‘Fig S3.csv’ contains the data associated with Fig. S3. The raw data is provided in columns 1-6 with column 1 being the time (in minutes) at which the weight was recorded. Columns 2-6 are the individual weights of each cuboid of PDMS (column 2), 0.25% PDMS-Brij L4 (column 3), 0.75% PDMS-Brij L4 (column 4), 1% PDMS-Brij L4 (column 5), and 1.5% PDMS-Brij L4 (column 6). Difference calculations are provided in columns 8-12, showing the difference from the measured starting weight. Columns 2-12 all have units of grams. Finally, column 14 reprises the time values with columns 15-19 are the mean percentage changes for PDMS and each PDMS-Brij L4 mix and columns 21-25 give the standard deviation values. Columns 15-25 all have units of percentage. Columns 7 and 13 are intentionally left blank for readability. ‘Fig S4.csv’ contains the data associated with Fig. S4. Columns 1-7 contain the data for the Fig. S4 plot. Column 1 is the time values for which the cosine of the contact angles, cos(theta) values, are provided for. The square root of the time values is given in column 2. Columns 3-5 are the calculated cosine of the contact angle values for 3 repeats. The mean and standard deviation of the cos(theta) values are provided in columns 6 and 7. The diffusivity calculation is given in columns 9-16. Columns 9 and 10 are components of the modified Young-Dupre equation (given in the supplementary file of the paper). Column 11 is the calculated values for the diffusion multiplied by time, the diffusion coefficient. The mean value for column 11 is calculated in column 12 alongside the mean time in column 13 which are both used to calculate the diffusion in column 14. The diffusion time, in seconds, is calculated for a length of 100 micrometres (column 15) and converted to hours in column 16. All constants used in the equations are provided in columns 18 and 19 which represent the names/units and the values respectively. Columns 8 and 17 are intentionally left blank for readability.