README File: 

Title: Loop-mediated isothermal amplification as a powerful tool for early diagnosis of hepatitis C virus.

Authors: Weronika Witkowska McConnell, Chris Davis, Suleman R. Sabir, Alice Garrett, Amanda Bradley-Stewart, Pawel Jajesniak, Julien Reboud, Gaolian Xu, Zhugen Yang, Rory Gunson, Emma C. Thomson, Jonathan M. Cooper.
 

This readme file aims to provide a guide to use the data associated with this publication, available here in the following folders : 

1. raw data of figure 1, figure 2 and figure S4
  1-1 raw data of figures from first batch of clinical samples tested in the double blind study
  1-2 raw data of figures from second batch of samples tested in the double blind study
  1-3 raw data of figures from third batch of samples tested in the double blind study


2. data of figure 3 
 
2-1, first measurement - analytical sensitivity based on fluorescence, 
2-1, second measurement (replicate) - analytical sensitivity based on fluorescence, 
2-2, third measurement (replicate) - analytical sensitivity based on fluorescence.
2-4, original gel blot image - analytical sensitivity based on gel electrophoresis
2-5, first original scanned image - analytical sensitivity based on nucleic-acid-based lateral flow strips,
2-6, second original scanned image (replicate) - analytical sensitivity based on nucleic-acid-based lateral flow strips,
2-7, third original scanned image (replicate) - analytical sensitivity based on nucleic-acid-based lateral flow strips.
 
3. data of figure S1, 
3.1, aligment file of sequences used for figure S1 diagram

4. Figure S2: Evaluation of novel backward loop primer in genotype 3 standard.
4, raw data of figure S2

5. Figure S3:Determination of time to positivity.
5, raw data of figure S3; explanation on how positive samples and the time to positivity were defined.

6. Data for Table S2: comparison between qPCR results and RT-LAMP on lateral flow devices for 40 clinical samples.

6-1, qRT-PCR Test1 - Samples P1-P5
6-2, qRT-PCR Test2 - Samples P6-P15
6-3, qRT-PCR Test3 - Samples P16-P30
6-4, qRT-PCR Test4 - Samples P31-P40
6-5, Lateral flow image Test 1 - Samples P1-P5
6-6, Lateral flow image Test 2 - Samples P1-P5
6-7, Lateral flow image Test 3 - Samples P6-P15
6-8, Lateral flow Image Test 4 Plate 1 - Samples P16-P20
6-9, Lateral flow image Test 4 Plate 2 - Samples P21-P30
6-10, Lateral flow image Test 5 - Samples P21-P40
6-11, Lateral flow image Test 7 Plate 2 - Samples P6-P15
6-12, Lateral flow image Test 7 Plate 1 - Samples P16-P20
6-13, Lateral flow image Test 7 Plate 3 - Samples P31-P40


7. Data for Figure S6: temperature measurements "Figure S6 temperature data.csv"
The first row contains column headers. The first column is the time of the measurement (starting at 0) in seconds, whilst the other columns are the temperature (in degrees celsius) measured at the three locations in the device.