In vitro evidence consistent with an interaction between wild type and a mutant SOD1 protein associated with canine degenerative myelopathy

Qi, Y., Montague, P., Loney, C., Campbell, C., Shafie, I., Anderson, T. and McLaughlin, M. (2019) In vitro evidence consistent with an interaction between wild type and a mutant SOD1 protein associated with canine degenerative myelopathy. [Data Collection]

Enlighten Publications URI: http://eprints.gla.ac.uk/id/eprint/191694

Collection description

The data sets are associated with four experimental approaches to investigate a mutant gene that had been cloned into a vector plasmid to generate fluorescent tagged (Cherry or GFP wildtype or mutant SOD1 proteins:
a. a collection of fluorescent images of cells to count aggregate positive cells (WT and Mutant (DM) with GFP or Cherry) (JPEGS format)
b. A collection of western blots that confirm the expression of the SOD1 fusion protein, the fluorescent protein only and loading control with b-actin. (Tiff format)
c. A native gel treated with NBT to display SOD1 activity. Corresponding western blots shown to confirm SOD1 fusion protein expression. (Tiff format)
d. Confocal images showing the co-expression of wild type and mutant fusion proteins within cellular aggregates. (Tiff format)
e. Western blot of immunoprecipitation (pull down) products supporting the co-precipitation of wildtype and mutant SOD1. (Tiff format)

College / School: College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation > Centre for Virus Research
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Date Deposited: 02 Aug 2019 13:11
URI: http://researchdata.gla.ac.uk/id/eprint/786

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Qi, Y., Montague, P., Loney, C., Campbell, C., Shafie, I., Anderson, T. and McLaughlin, M. (2019); In vitro evidence consistent with an interaction between wild type and a mutant SOD1 protein associated with canine degenerative myelopathy

University of Glasgow

DOI: 10.5525/gla.researchdata.786

Retrieved: 2019-11-14

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