E-MTAB-6265 - Transcription profiling by RNA-seq of murine colon tumours after in situ depletion of Nuak1

Port, J., Hedley, A. and Murphy, D. (2018) E-MTAB-6265 - Transcription profiling by RNA-seq of murine colon tumours after in situ depletion of Nuak1. [Data Collection]

Enlighten Publications URI: http://eprints.gla.ac.uk/id/eprint/157305

Collection description

The aim of the project is to determine the impact of NUAK1 depletion on the transcriptome of in situ colon tumours. Tumours were initiated in Villin-CRE-ER;APC-floxed;DI-shNUAK1 mice, followed by treatment for 1 week with dextran sodium sulphate. 68 days after tumour initiation, NUAK1 shRNAs were induced by doxycycline administration (via gavage), or left un-induced. 6 tumours were harvested from NUAK1 shRNA expressing mice and 6 from controls. RNA was isolated using Qiagen RNEasy and analyzed by Illumina paired-end RNA-SEQ

College / School: College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
Date Deposited: 28 Mar 2019 11:55
Funder's Name: European Commission (EC), European Commission (EC), British Lung Foundation (BLF)
URI: http://researchdata.gla.ac.uk/id/eprint/763

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Port, J., Hedley, A. and Murphy, D. (2018); E-MTAB-6265 - Transcription profiling by RNA-seq of murine colon tumours after in situ depletion of Nuak1

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http://researchdata.gla.ac.uk/763

Retrieved: 2020-10-25