Zinc uptake promotes myoblast differentiation via Zip7 transporter and activation of Akt signalling transduction pathway

Mnatsakanyan, H., Sabater i Serra, R., Rico, P. and Salmeron-Sanchez, M. (2018) Zinc uptake promotes myoblast differentiation via Zip7 transporter and activation of Akt signalling transduction pathway. [Data Collection]

Enlighten Publications URI: http://eprints.gla.ac.uk/id/eprint/168423
Not all data is available to download from this page. Usually this is because the dataset is too large or it is restricted in some way.

Collection description

Myogenic regeneration occurs through a chain of events beginning with the output of satellite cells from quiescent state, formation of competent myoblasts and later fusion and differentiation into myofibres. Traditionally, growth factors are used to stimulate muscle regeneration but this involves serious off-target effects, including alterations in cell homeostasis and cancer. In this work, we have studied the use of zinc to trigger myogenic differentiation. We show that zinc promotes myoblast proliferation, differentiation and maturation of myofibres. We demonstrate that this process occurs through the PI3K/Akt pathway, via zinc stimulation of transporter Zip7. Depletion of zinc transporter Zip7 by RNA interference shows reduction of both PI3K/Akt signalling and a significant reduction of multinucleated myofibres and myotubes development. Moreover, we show that mature myofibres, obtained through stimulation with high concentrations of zinc, accumulate zinc and so we hypothesise their function as zinc reservoirs into the cell.

College / School: College of Science and Engineering > School of Engineering > Biomedical Engineering
Date Deposited: 05 Nov 2018 10:41
Funder's Name: European Research Council (ERC), Engineering and Physical Sciences Research Council (EPSRC)
URI: http://researchdata.gla.ac.uk/id/eprint/690

Available Files

Read me

Repository Staff Only: Update this record

Mnatsakanyan, H., Sabater i Serra, R., Rico, P. and Salmeron-Sanchez, M. (2018); Zinc uptake promotes myoblast differentiation via Zip7 transporter and activation of Akt signalling transduction pathway

University of Glasgow

DOI: 10.5525/gla.researchdata.690

Retrieved: 2020-09-28

Downloads

Downloads per month over past year