This folder contains data that underpin the work described in: Stacking-induced fluorescence increase reveals allosteric interactions through DNA, Nucleic Acids Research. 10.1093/nar/gky887 Michael J. Morten, Sergio G. Lopez, I. Emilie Steinmark, Aidan Rafferty and Steven W.* Magennis School of Chemistry, WestCHEM, University of Glasgow, Joseph Black Building, University Avenue, Glasgow, G12 8QQ, UK *corresponding author The dataset comprises: Time-resolved fluorescence (TCSPC) data (Fig. 2b, Fig. 2d and Fig 4) Time traces from single-molecule movies (Fig. 3)