Dataset associated with study entitled: 
A rapid variant-tolerant reverse transcription loop-mediated isothermal amplification assay for point of care detection of HIV-1

This document provides a description of the data and the link to the figures in the manuscript, which contains the methods used to obtain and analyse them. 

Figure 2. “Specifiticy.xls” 
contains 2 sheets for a repeat of the same experiment. 
The first row of each sheet is column headers. 
Column 1 (header “cycle”) contains the time values. Each row is an increment of 1min. 
Columns 2-10 contain the fluorescence intensity values for the different organisms tested (one per column).

Figure 3. “Sensitivity.xls”
Contains 3 sheets of data from repeated experiments
The first row of each sheet is column headers. 
Column 1 (header “cycle”) contains the time values. Each row is an increment of 1min. 
Columns 2-6 contain the fluorescence intensity values for the different concentrations of template tested (one per column). The last column (“NTC”) corresponds to a concentration of 0. 

Table 1. “Comparison of conventional  and novel  LAMP for detecting wild and mutated templates”
Contains 3 sheets of data from repeated experiments
The first row of each sheet is column headers. 
Column 1 (header “cycle”) contains the time values. Each row is an increment of 1min. 
Columns 2-30 contain the fluorescence intensity values for the components of the reaction tested (one per column).

Table 2. “LOD detection”
Contains 10 sheets of data from repeated experiments
The first row of each sheet is column headers. 
Column 1 (header “cycle”) contains the time values. Each row is an increment of 1min. 
Columns 2-6 contain the fluorescence intensity values for each concentration of template tested (one per column).

Table 3 is built with three datasets, one for each technique used in the experiments. 
They are all sharing a single format where 
The first row of each sheet is column headers. 
Column 1 (header “cycle”) contains the time values. Each row is an increment of 1min. 
Columns 2-83 contain the fluorescence intensity values for sample (one per column). The numbers identify the samples across the datasets. 
The three datasets are : 
“Samples test of conventional RT- LAMP.xls” for the detection with conventional RT-LAMP
“Samples test of HIV RT-qPCR” for RT-qPCR.xls”
And “Samples test of novel RT- LAMP.xls“ for the new variant-tolerant assay. 

Table 4 “Lateral flow strip test of the RT-LAMP products of clinical sample.xls”
The first row of each sheet is column headers. 
Column 1 (header “cycle”) contains the time values. Each row is an increment of 1min. 
Columns 2-48 contain the fluorescence intensity values for each sample (one per column). These are different samples from those in Table 3 (the identifiers should not be correlated).